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What is the TZM-BL reporter cell line assay?

Among the pseudovirus-based assays, the standardized TZM-bl reporter cell line assay ( Platt et al., 2009) is widely used as a validated standard platform for HIV-1 vaccine and drug development. The TZM-bl cell line, also known as the JC. 53bl-13 cell line, is a genetically modified HeLa cell line that expresses CD4 and CCR5 to allow HIV entry.

Do TZM-BL cells express a 48 kDa nonglycosylated protein?

The cell line is highly permissive to infection by most strains of HIV, SIV, and SHIV. Here we demonstrated that TZM-bl cells express a 48 kDa non-glycosylated protein (p48) recognized by broadly neutralizing monoclonal antibody (mAb) 2F5 targeting the ELDKWA (aa 669-674) epitope of gp41TM of HIV-1.

What is TZM-BL Clone 13?

Reagent: TZM-bl Cells Catalog Number: 8129 Lot Number: 150078 Release Category: C Provided: 1 ml/vial (5.5 x 106 cells/vial). Viability is 96%. Cell Type: TZM-bl, previously designated JC53-bl (clone 13) is a HeLa cell line. Propagation Medium: DMEM (90%), 10% FBS, 100 units of Penicillin and 0.1 mg/ml of Streptomycin.

What is TZM BL indicator?

Description: The TZM-bl indicator cell line enables simple and quantitative analysis of HIV using either β-gal or luciferase as a reporter. It is maximally sensitive to HIV infection by including DEAE-dextran in the infection medium. Special Characteristics:

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